Center Members
Colon Cancer Tissue Microarray Now Available
The Cancer Diagnosis Program is pleased to announce the availability of a Colon Cancer Tissue Microarray that contains 366 primary colon cancers along with appropriate diverticulitis, adenomatous polyps and normal colon mucosa as controls. Designed by a biostatistician, it is sufficiently robust to allow for critical testing of a marker’s prevalence in colon carcinoma as well as its association with clinical outcome. The accompanying brochure describes the clinical annotation documented in the TMA. Further information with instructions about applying for the TMA, its cost and the data and statistical design manuals are available at:
http://cdp.nci.nih.gov.
This technology is designed for examination of
associations of markers with tumor
stage, clinical outcome, and other
clinico-pathologic variables in Stages I–IV
Each tissue microarray
(TMA) set consists of
-
invasive colon carcinoma
- normal colon epithelium
- adjacent to the cancer: N=34
- diverticulitis, no cancer: N=40
-
adenomatous polyps
- controls
-
normal non-colon
tissues: N=4
- cell lines: N=6
The TMA was designed by NCI statisticians for high statistical power for comparisons
of marker values
- between colon cancers of different stages, or
- between colon cancers that recurred within a few years of diagnosis versus colon
cancers that remained recurrence free. For example, there is 80% power to detect
- difference in prevalence
rate for a binary marker
of about 0.30 between
Stage I and
Stage IV cancer
- difference in prevalence
rate of about 0.35
between Stage II colon
cancers that recurred
within 5 years versus
those that did not recur
within 5 years.
Each array is created in
quadruplicate to address
possible tumor
heterogeneity.
- Four cores are taken
from each case block,
with one of those cores
placed in each of four
replicate array blocks.
-
Information for invasive
colon carcinoma cases:
-
race, gender and age
at diagnosis
- year of diagnosis
- primary site within colon
- TNM stage (AJCC 5th edition)
- number of positive nodes and total number examined
- initial treatment— only Y/N: surgery, chemotherapy, radiation
- histology, grade, lymphovascular invasion, status of margins
- recurrence and time to recurrence
- vital status
- cancer status at last follow-up
Information for adenomatous polyp cases:
-
gender
- age at diagnosis
- year of diagnosis
- polyp type
- degree of dysplasia
- gross size of polyp
Application instructions for this TMA are available at:
http://cdp.nci.nih.gov/
For additional information, contact:
Cindy Mattingly
Information Management Services, Inc.
12501 Prosperity Drive, Suite 200
Silver Spring, MD 20904
301.680.9770
tissueresources@imsweb.com
